Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human MEK2 around the phosphorylation site of threonine 394.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P36507
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:10000 IP: Various Dilution
Specificity Sensitivity:
MEK2 (Ab-394) antibody detects endogenous levels of MEK2 protein around threonine 394.
References:
Crews C M, et al. (1992) Science. 258:478-480.
Alessi D R, et al. (1994) EMBO J. 13:1610-1619.
Rosen L B, et al. (1994) Neuron. 12:1207-1221.
Cowley S, et al. (1994) Cell. 77:841-852.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK2 (Ab-394) antibody (#B7149).
Western blot analysis of extracts from ovary cancer cells using MEK2 (Ab-394) antibody (#B7149).