• Catalog Number:

  E1A8179A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human C-RAF around the phosphorylation site of Ser301.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide.

• Reactivity:

  H:S301, M:S301, R:S301

• SwissProtNo:

  P04049

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Alter Name:

  RAF proto-oncogene serine/threonine-protein kinase EC=2.7.11.1, Proto-oncogene c-RAF,cRaf, RAF, RAF1

• Specificity Sensitivity:

  C-RAF (Phospho-Ser301) Antibody detects endogenous levels of C-RAF only when phosphorylated at Ser301.

• References:

  Young A.C., Rapp U.R. Nucleic Acids Res. 14:1009-1015(1986) Sugano S.Nat. Genet. 36:40-45(2004) NIEHS SNPs program Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases

P-peptide - + Western blot analysis of extracts from HuvEc, using C-RAF (Phospho-Ser301) Antibody.