• Catalog Number:

  E011321-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Smad1 around the phosphorylation site of serine 465 (I-S-S-V-SP).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q15797

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  Smad1 (Phospho-Ser465) Antibody detects endogenous levels of Smad1 only when phosphorylated at serine 465.

• References:

  Hiroaki Seto, et al. (2004) J Clin Invest. March 1; 113(5): 718–726 Linyu Li, et al. (2004) Mol Cell Biol. January; 24(2): 856–864. Claire Nourry, et al. (2004) BMC Cell Biol. 5: 20

Western blot analysis of extract from 293 cells, untreated or treated with PMA (200nM, 30min), using Smad1 (Ab-465) Antibody (E021321, Lane 1 and 2) and Smad1 (Phospho-Ser465) Antibody (E011321, Lane 3 and 4).