Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human MARCKS around the phosphorylation site of serine 158(R-F-SP-F-K).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P29966
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000
Other Names:
80K-L protein; MACS; MARCS; myristoylated alanine-rich C-kinase substrate; PKCSL; PRKCSL; protein kinase C substrate, 80 kDa protein, light chain
Specificity Sensitivity:
MARCKS (Ab-158) antibody detects endogenous levels of total MARCKS protein.
References:
Harlan D.M., J. Biol. Chem. 266:14399-14405(1991).
Sakai K., Genomics 14:175-178(1992).
Mungall A.J., Nature 425:805-811(2003).
Peptide - +
Western blot analysis of extracts from mouse brain cells, using MARCKS (Ab-158) antibody.
Immunofluorescence analysis of A549 cells, using MARCKS (Ab-158) antibody.