• Catalog Number:

  E1A9003A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ATF4 around the phosphorylation site of serine 219.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human; Mouse

• SwissProtNo:

  P18848

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  Activating transcription factor 4; activating transcription factor 4 (tax-responsive enhancer element B67); ATF4; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2; CREB2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67; TXREB

• Specificity Sensitivity:

  ATF-4 (Phospho-Ser219) Antibody detects endogenous levels of ATF4 only when phosphorylated at serine 219.

• References:

  Tsujimoto A., J. Virol. 65:1420-1426(1991). Karpinski B.A., Proc. Natl. Acad. Sci. U.S.A. 89:4820-4824(1992). Karpinski B.A., Proc. Natl. Acad. Sci. U.S.A. 89:4820-4824(1992).

• All Sites:

  Human: Ser219; Mouse: Ser218

Western blot analysis of extracts from COLO205 cells, using ATF-4 (Phospho-Ser219) Antibody.