Rabbit IgG in phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human β-Catenin around the phosphorylation site of Threonine 41/Serine 45.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P35222
Applications:
WB: 1:500~1:1000 ELISA: 1: 20000
Specificity Sensitivity:
β-Catenin(Ab-41/45) Antibody detects endogenous levels of total β-Catenin protein.
References:
Provost E, et al. (2003)J Biol Chem 22; 278(34): 31781-9.
Sakanaka C , et al. (2003)J Biochem (Tokyo); 132(5): 697-703.
Amit S, et al. (2002) Genes Dev; 16(9): 1066-76.
Liu C, et al. (2002) Cell; 108(6): 837-47.
Provost E, et al. (2005) Oncogene; 24(16): 2667-76.
Western blot analysis of extracts from SW626 cell using β-Catenin (Ab-41/45) Antibody(#B7024) and β-Catenin(Phospho-Thr41/Ser45) Antibody( #11116): Line1: Using #B7024; Line2: Using #B7024 preincubated with synthesized peptide.