Rabbit IgG in phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
This antiserum was produced against synthesized non-phosphopeptide derived from human CREB around the phosphorylation site of serine 129 or serine 133(S-R-R-P-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P16220
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000
Specificity Sensitivity:
CREB (Ab-129/133) antibody detects endogenous levels of total CREB protein.
References:
Xing J, et al. (1998) Mol Cell Biol 18(4): 1946-55.
Tan Y, et al.( 1996) EMBO J; 15(17): 4629-42.
Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385.
Mayo LD, et al. (2001) Biol Chem; 276(27): 25184-9.
Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (Ab-129/133) antibody (#B7053).
Western blot analysis of extracts from HeLa cells treated with UV, using CREB (Ab-129/133) antibody (#B7053).