Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human SP1 around the phosphorylation site of threonine 739 (T-A-TP-P-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
SP1 (Ab-739) antibody detects endogenous levels of total SP1 protein.
References:
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Haggart M.H., J. Biol. Chem. 275:38067-38072(2000).
Kadonaga J.T., Cell 51:1079-1090(1987).
Peptide - +
Western blot analysis of extracts from HT-29 cells, using SP1 (Ab-739) antibody.
Immunofluorescence analysis of HepG2 cells, using SP1 (Ab-739) antibody.