Western blot analysis of extracts of various cell lines, using NTRK2 Antibody (E912011) at 1: 3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H + L) (AS014) at 1: 10000 dilution. Lysates / proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.