• Catalog Number:

  E912011

• Amount:

  100ul

• Form of Antibody:

  Store at -20 ℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Experimental method:Western blotting Immunohistochemistry Positive sample:K-562, Rat brain Cell localization:Cell membrane, Endosome membrane, Single-pass type I membrane protein

• Storage/Stability:

  Store at -20°C . Avoid freeze / thaw cycles.

• class="eg_cn_title"Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 41-433 of human TrkB (NP_001007098.1).Sequence:SRIWCSDPSPGIVAFPRLEPNSVDPENITEIFIANQKRLEIINEDDVEAYVGLRNLTIVDSGLKFVAHKAFLKNSNLQHINFTRNKLTSLSRKHFRHLDLSELILVGNPFTCSCDIMWIKTLQEAKSSPDTQDLYCLNESSKNIPLANLQIPNCGLPSANLAAPNLTVEEGKSITLSCSVAGDPVPNMYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVKGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYTLIAKNEYGKDEKQISAHFMGWPGIDDGANPNYPDVIYEDYGTAANDIGDTTNRSNEIPSTDVTDKTGREHLSV

• Purification:

  Affinity purified

• SwissProtNo:

  Q16620Alias:NTRK2; GP145-TrkB; TRKB; trk-B; OBHD; BDNF / NT-3 growth factors receptor Reactive species:Human, Rat

• Source:

  Rabbit

• Applications:

  WB, IHCRecommended dilution ratio: WB 1: 500-1 : 2000 IHC 1: 50-1: 200 Theoretical molecular weight:35kDa / 53kDa / 59kDa / 60kDa / 81kDa / 91kDa / 93kDa Actual molecular weight:110kDa

Immunohistochemistry of paraffin-embedded human lung cancer using NTRK2 antibody (E912011) at dilution of 1: 200 (40x lens).

Western blot analysis of extracts of various cell lines, using NTRK2 Antibody (E912011) at 1: 3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H + L) (AS014) at 1: 10000 dilution. Lysates / proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.