• Catalog Number:

  E1C604-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit polyclonal (IgG) 1mg/ml in PBS, pH 7.4 with 50% glycerol, 0.05% sodium azide. Frozen liquid.

• Storage/Stability:

  Stable for 1 year at -20C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4C for 3 months.

• class="eg_cn_title"Immunogen:

  Purified recombinant human GAPDH.

• Purification:

  Affinity purified

• Reactivity:

  Human, Mouse, Rat, others

• Applications:

  Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows: Western blot at dilution of 1:1000-4000, Immunofluorescence staining at 1:100-400 dilutions, IHC at 1:100-400 dilutions. It is very useful as a loading control for quantitative western blotting. The antibody recognizes a 37kDa band in a Western Blot analysis.

• References:

  Cummings J, et al. (2005) Br J Cancer; 92 (3): 532-538.

Western blot analysis of GAPDH expression in 293A (A) and Hela (B) whole cell lysates and mouse kidney tissue extract (C) using GAPDH polyclonal antibody (E1C604).