• Catalog Number:

  E1A0855A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CDC25B around the phosphorylation site of serine 353 (R-R-SP-V-T).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P30305

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  CDC25HU2, CDC25M2, Dual specificity phosphatase Cdc25B, EC 3.1.3.48, M-phase inducer phosphatase 2, MPIP2

• Specificity Sensitivity:

  CDC25B (Phospho-Ser353) antibody detects endogenous levels of CDC25B only when phosphorylated at serine 353.

• References:

  Sanae Uchida, J. Cell Sci., Jun 2004; 117: 3011 - 3020. Christina Karlsson, J. Cell Biol., Aug 1999; 146: 573. Stéphanie Dutertre, J. Cell Sci., May 2004; 117: 2523 - 2531.

• All Sites:

  Human: Ser353

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using CDC25B (Phospho-Ser353) antibody. Western blot analysis of extracts from COS7 cells, treated with etoposide (25uM, 24hours), using CDC25B (Phospho-Ser353) antibody.