• Catalog Number:

  E20-60348

• Amount:

  100ul

• Form of Antibody:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. .

• Storage/Stability:

  Stability: -20°C/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218. AA range171-220

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human,Mouse

• Source:

  Rabbit

• Applications:

  WB: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

• Other Names:

  CD28; T-cell-specific surface glycoprotein CD28; TP44; CD antigen CD28

• Specificity Sensitivity:

  Phospho-CD28 (Y218) Polyclonal Antibody detects endogenous levels of CD28 protein only when phosphorylated at Y218.

Enzyme-Linked Immunosorbent Assay (Phospho- ELISA) for Immunogen Phospho- peptide (Phospho-left) and Non- Phospho- peptide (Phospho-right), using CD28 (Phospho- Tyr218) Antibody

WB analysis of lysates from HepG2 cells treated with nocodazole 1ug/ml 16h, using CD28 (Phospho-Tyr218) Antibody. The lane on the right is blocked with the phospho peptide.