Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human p95/NBS1 around the phosphorylation site of serine 343 (S-L-SP-Q-G).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human
SwissProtNo:
O60934
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
p95/NBS1 (phospho-Ser343) antibody detects endogenous levels of p95/NBS1 only when phosphorylated at serine 343.
References:
Hsu HL, et al (2005) Oncogene; 24(31): 4956-64.
Falck J, et al. (2005) Nature; 434(7033): 605-11.
Buscemi G, et al. (2004) Oncogene; 23(46): 7691-700.
Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A; 101(33): 12130-5.
Western blot analysis of extracts from Jurkat cells using p95/NBS1 (Ab-343) antibody (E021058, Lane 1 and 2) and p95/NBS1 (phospho-Ser343) antibody (E011057, Lane 3 and 4).