• Catalog Number:

  E20-60051

• Amount:

  100ul

• Form of Antibody:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. .

• Storage/Stability:

  Stability: -20°C/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized peptide derived from human CD3 zeta around the phosphorylation site of Tyr142. AA range111-160

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope- specific immunogen.

• Reactivity:

  Human, Mouse, Rat, Monkey

• Source:

  Rabbit

• Applications:

  WB: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

• Other Names:

  CD247; CD3Z; T3Z; TCRZ; T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD antigen CD247

• Specificity Sensitivity:

  Phospho-CD3 ζ (Y142) Polyclonal Antibody detects endogenous levels of CD3 ζ protein only when phosphorylated at Y142.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phospho- peptide (Phospho-left) and Non- Phospho- peptide (Phospho-right), using CD3 zeta (Phospho-Tyr142) Antibody

WB analysis of lysates from Jurkat cells treated with UV 15', using CD3 zeta (Phospho-Tyr142) Antibody. The lane on the right is blocked with the phospho peptide.