• Catalog Number:

  E1A7050A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Cortactin around the phosphorylation site of tyrosine 466 (P-V-YP-E-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human; Mouse; Rat

• SwissProtNo:

  Q14247

• Applications:

  WB: 1:500~1:1000 IHC:1:50~1:100 ELISA: 1:20000

• Alter Name:

  Amplaxin, CTTN, EMS1, Oncogene EMS1, SRC8, Src substrate cortactin

• Specificity Sensitivity:

  Cortactin (phospho-Tyr466) antibody detects endogenous levels of Cortactin only when phosphorylated at tyrosine 466.

• References:

  Julie A. Head, Mol. Biol. Cell, Aug 2003; 14: 3216. Cherry Luo, J. Biol. Chem., Oct 2006; 281: 30081 - 30093. Jianwei Zhu, J. Biol. Chem., Apr 2007; 10.1074. David Hattan, J. Biol. Chem., Oct 2002; 277: 38596 - 38606.

• All Sites:

  Human: Tyr470; Mouse: Tyr466; Rat: Tyr428

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cortactin (phospho-Tyr466) antibody.

Western blot analysis of extracts from cos-7cells using Cortactin (phospho-Tyr466) antibody (Line 1 and 2).