• Catalog Number:

  E1PA1S005

• Amount:

  50ul

• Form of Antibody:

  Liquid. The product is 1.0 mg/ml in 20mM HEPES, pH7.5, 100mM NaCl, 10mM beta-ME, 10% glycerol, 0.5mM EDTA.

• Storage/Stability:

  Store at -20°C. -80°C for long-term storage. Avoid exposure to light. Avoid repeated freezing and thawing.

• Source:

  Human protein tyrosine phosphatase (residues 1-322), expressed in E. coli.

• Applications:

  The protein is suitable for study tyrosine phosphatase kinetics and substrate specificity.

• Specificity Sensitivity:

  ~50 μmol/min/mg at 30°C using 0.15 mM of phosphopeptide substrate based on EGFR residues 988-998 in 50 mM HEPES, pH 7.2, 1 mM EDTA, 1 mM DTT, 0.05% NP-40, and 0.2 μg/μl bovine serum albumin.

• References:

  Ukkola O., et al. Obes. Res. 13:829-834(2005) Puius Y.A., et al.Proc. Natl. Acad. Sci. U.S.A. 94:13420-13425(1997)

Coomassie Blue stained SDS-PAGE(12% acrylamide) of PTP1B, PTPase (E1PA1).