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Catalog Number:
E1GA1-100 Amount:
1000μg Form of Antibody:
Liquid. The product is 1 mg/ml in 10 mM Phosphate buffered saline, pH 7.4 containing, 10% glycerol and 0.1% sodium azide as preservative. Storage/Stability:
Store at -20°C. -80°C for long-term storage. Avoid exposure to light. Avoid repeated freezing and thawing. Source:
Recombinant expressed in E. coli Applications:
The protein is suitable as control reagent for EGFP expression studies or as labeling reagent. Applications include: Flow cytometers, fluorescence microscopy, and microinjection, antibody purification, etc. at 1:100 dilution. Maximum absorption wavelength at 488 nm and emission wavelength at 507 nm. Specificity Sensitivity:
Under optimal conditions, 1 mg EGFP-rProtein A will bind approximately 5 mg IgG. Optimal binding of EGFP-rProtein A to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.
293T cells were stained with mouse anti-beta-tubulin antibody (E1C601) and detected with EGFP-rProtein A (Green).