Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Tsc2 around the phosphorylation site of threonine 1462.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P49815
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity Sensitivity:
Tsc2 (Ab-1462) antibody detects endogenous levels of total Tsc2 protein.
References:
Cai SL, et al. (2006) J Cell Biol 173, 279-89.
Roux PP, et al. (2004) Proc Natl Acad Sci USA 101, 13489-94.
Li Y, Inoki K, Vacratsis P, Guan KL (2003) J Biol Chem 278, 13663-71.
Manning BD, et al. (2002) Mol Cell 10, 151-62.
Goncharova, E. et al. (2002) J. Biol. Chem. 277, 30958-30967.
Aicher, L. D. et al. (2001) J. Biol. Chem. 276, 21017-21021.
Peptide - +
Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using Tsc2 (Ab-1462) antibody (E022050).