Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Ephrin-B1 around the phosphorylation site of tyrosine 317 (P-H-YP-E-K).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Ephrin-B1 (Ab-317) antibody detects endogenous levels of total Ephrin-B1 protein.
References:
Beckmann M.P., EMBO J. 13:3757-3762(1994).
Ross M.T., Nature 434:325-337(2005).
Zhang Z., Protein Sci. 13:2819-2824(2004).
Brueckner K., Neuron 22:511-524(1999).
Western blot analysis of extracts from COS7 cells, using Ephrin-B1 (Ab-317) antibody.