Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Keratin 8 around the phosphorylation site of serine 73 (L-L-SP-P-L).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
P05787
Applications:
IHC: 1:50~1:100
Specificity Sensitivity:
Keratin 8 (Ab-73) antibody detects endogenous levels of total Keratin 8 protein.
References:
Nakamichi I, et al. (2002) Mol Biol Cell; 13(10): 3441-3451.
Prochasson P, et al. (2002) Nucleic Acids Res; 30(15): 3312-3322.
Pankov R, et al. (1994) Proc Natl Acad Sci U S A; 91(3): 873-877.
Thorey IS, et al. (1993) Mol Cell Biol; 13(11): 6742-6751.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 8 (Ab-73) antibody (E021307).