Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Synuclein around the phosphorylation site of serine 129 (M-P-SP-E-E).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
P37840
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Other Names:
NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, SYN, SYUA, alpha-synuclein, synuclein, alpha
Specificity Sensitivity:
Synuclein (Phospho-Ser129) antibody detects endogenous levels of Synuclein only when phosphorylated at serine 129.
References:
Natalia Ninkina, Mol. Cell. Biol., Nov 2003; 23: 8233 - 8245.
Julie Lotharius, J. Biol. Chem., Oct 2002; 277: 38884 - 38894.
Deniz Kirik, PNAS, Mar 2003; 100: 2884.
Zhong-Zong Pan, AACR Meeting Abstracts, Mar 2004; 2004: 1178.
All Sites:
Human: Ser129; Mouse: Ser129
Western blot analysis of extracts from mouse brain cells, using Synuclein (Phospho-Ser129) antibody.