• Catalog Number:

  E021122-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human IκB-α around the phosphorylation site of serine 32/36 (H-D-SP-G-L- D-SP -M-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P25963

• Applications:

  WB:1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200

• Specificity Sensitivity:

  IκB-α (Ab-32/36) antibody detects endogenous levels of total IκB-α protein.

• References:

  Mattioli I, et al. (2004)J Immunol; 172(10): 6336-44. Courtois G, et al. (2003)J Clin Invest; 112(7): 1108-15. Nair A, et al. (2003) Oncogene; 22(1): 50-8. Fan C, et al. (2002)J Cell Sci; 115(Pt 24): 4843-53. Schubert SY, et al. (2002)FASEB J; 16(14): 1931-3.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IκB-α (Ab- 32/36) antibody (E021122).

Western blot analysis of extracts from MCF7 cells using IκB-α (Ab-32/36) antibody (E021122).