• Catalog Number:

  E1A0058A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Caspase 6 around the phosphorylation site of serine 257 (K-V-SP-Q-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P55212

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  EC 3.4.22.-, ICE6, MCH2, apoptotic protease Mch-2, caspase-6

• Specificity Sensitivity:

  Caspase 6 (Phospho-Ser257) antibody detects endogenous levels of Caspase 6 only when phosphorylated at serine 257.

• References:

  Lijun Zhu, Mol. Cancer Ther., May 2005; 4: 787 - 798. Michael H. Cardone, Science, Nov 1998; 282: 1318. Carlos Caulín, J. Cell Biol., Sep 1997; 138: 1379.

• All Sites:

  Human: Ser257; Mouse: Ser239; Rat: Ser240

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Caspase 6 (Phospho-Ser257) antibody. Western blot analysis of extracts from 293 cells treated with Etoposide (25uM, 60min), using Caspase 6 (Phospho-Ser257) antibody.

P-peptide - + Immunofluorescence analysis of HuvEc cells, using Caspase 6 (Phospho-Ser257) antibody.