Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human FLT3 around the phosphorylation site of tyrosine 591 (Y-F-YP-V-D).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human
SwissProtNo:
P36888
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000
Specificity Sensitivity:
FLT3 (Phospho-Tyr591) antibody detects endogenous levels of FLT3 only when phosphorylated at tyrosine 591.
References:
Irish JM, et al. (2007) Blood 109, 2589-96.
Heiss E, et al. (2006) Blood 108, 1542-50.
Shurin, M.R. et al. (1998) Cytokine Growth Factor Rev. 9, 37-48.
Naoe, T. et al. (2001) Cancer Chemother. Pharmacol. 48 Suppl1, S27-S30.
Namikawa, R. et al. (1996) Stem Cells 14, 388-395.
Zhang, S. et al. (1999) J. Leukoc. Biol. 65, 372-380.
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using FLT3 (Phospho-Tyr591) Antibody (E012078).