• Catalog Number:

  E1A7163B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human NF-κB p65 around the phosphorylation site of serine 311 (F-K-SP-I-M).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q04206

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Alter Name:

  NFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, Transcription factor p65, p65

• Specificity Sensitivity:

  NF-κB p65 (Ab-311) antibody detects endogenous levels of total NF-κB p65 protein.

• References:

  Baeuerle, P.A. and Henkel, T. (1994) Annu. Rev. Immunol. 12, 141-179. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20. Haskill, S. et al. (1991) Cell 65, 1281-1289. Thompson, J.E. et al. (1995) Cell 80, 573-582.

Western blot analysis of extracts from Hela cells untreated or treated with TNF-alpha (20 ng/ml), using 4E-BP1 (Ab-45) antibody (#B7163, Line1 and 2).