• Catalog Number:

  E012046-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human LKB1 around the phosphorylation site of serine 428 (R-L-SP-A-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, mouse, rat

• SwissProtNo:

  Q15831

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  LKB1 (phospho-Ser428) antibody detects endogenous levels of LKB1 only when phosphorylated at serine 428.

• References:

  Shaw, R.J. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 3329-3335. Woods, A. et al. (2003) Curr. Biol. 13, 2004-2008. Karuman P, et al. (2001) Mol Cell 7, 1307-19 Nezu, J. et al. (1999) Biochem. Biophys. Res. Commun. 261, 750-755 Hemminki, A. et al. (1998) Nature 391, 184-187

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LKB1 (phospho- Ser428) antibody (E012046).

Western blot analysis of extracts from CV-1 cells treated with forsklin. Left: Using LKB1 (phospho- Ser428) antibody (E012046). Right: Using the same antibody preincubated with synthesized peptide.