• Catalog Number:

  E1A7069A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Elk1 around the phosphorylation site of serine 389 (P-R-SP-P-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P19419

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IP: Various Dilution ELISA: 1:10000

• Other Names:

  ELK1; ETS-domain protein Elk-1; ETS-domain protein ELK-1

• Specificity Sensitivity:

  Elk1 (Phospho-Ser389) antibody detects endogenous levels of Elk1 only when phosphorylated at serine 389.

• References:

  Rao V.N., Science 244:66-70(1989). Harindranath N., Gene 221:215-224(1998). Aryee D.N.T., Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.

• All Sites:

  Human: Ser389; Mouse: Ser390; Rat: Ser388

Western blot analysis of extracts from Jurkat cells treated with UV (15mins) and HeLa cells treated with paclitaxel (1uM, 24hours), using Elk1 (Phospho-Ser389) antibody.