• Catalog Number:

  E012033-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PDGFR beta around the phosphorylation site of tyrosine 751 (V-D-YP-V-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P09619

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  PDGFR beta (phospho-Tyr751) antibody detects endogenous levels of PDGFR beta only when phosphorylated at tyrosine 751.

• References:

  Bergsten, E. et al. (2001) Nat. Cell Biol. 3, 512-516 Kovalenko M, et al. (2000) J Biol Chem 275, 16219-26 Ramalingam, K. et al. (1995) Bioorg. Med. Chem. 3, 1263-1272 Wennström S, et al. (1994) Oncogene 9, 651-60 Kazlauskas A, Kashishian A, Cooper JA, Valius M (1992) Mol Cell Biol 12, 2534-44

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDGFR beta (phospho-Tyr751) antibody (E012033).

Western blot analysis of extracts from NIH/3T3 cells treated with PDGF. Left: Using PDGFR beta (phospho- Tyr751) antibody (E012033). Right: Using the same antibody preincubated with synthesized peptide.