• Catalog Number:

  E1A7256A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ZAP-70 around the phosphorylation site of tyrosine 493 (S-Y-YP-T-A).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P43403

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Other Names:

  70 kDa zeta-associated protein; EC 2.7.10.2; kinase ZAP70; SRK; Syk-related tyrosine kinase; ZA70; ZAP-70; ZAP70

• Specificity Sensitivity:

  ZAP-70 (Phospho-Tyr493) antibody detects endogenous levels of ZAP-70 only when phosphorylated at tyrosine 493.

• References:

  Chan A.C., Cell 71:649-662(1992). Kuroyama H., Biochem. Biophys. Res. Commun. 315:935-941(2004). Hillier L.W., Nature 434:724-731(2005).

• All Sites:

  Human: Tyr493; Mouse: Tyr492; Rat: Tyr488

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human thymus gland tissue using ZAP-70 (Phospho-Tyr493) antibody. Western blot analysis of extracts from Jurkat cells, using ZAP-70 (Phospho-Tyr493) antibody.