• Catalog Number:

  E012030-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human P53 around the phosphorylation site of serine 20 (T-F-SP-D-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P04637

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  P53 (phospho-Ser20) antibody detects endogenous levels of P53 only when phosphorylated at serine 20.

• References:

  Wang H, et al. (2008) J Biol Chem 283, 2564-74 Mayo LD, et al. (2005) J Biol Chem 280, 25953-9 Thompson T, et al. (2004) J Biol Chem 279, 53015-22 Hirao, A. et al. (2000) Science 287, 1824-1827. Levine, A.J. (1997) Cell 88, 323-331. Shieh, S.Y. et al. (1997) Cell 91, 325-334.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P53 (phospho- Ser20) antibody (E012030).

Western blot analysis of extracts from COS7 cells treated with UV. Left: Using P53 (phospho-Ser20) antibody (E012030). Right: Using the same antibody preincubated with synthesized peptide.