• Catalog Number:

  E011132-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 780 (T-L-SP-P-I).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P06400

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  Rb (phospho-Ser780) antibody detects endogenous levels of Rb only when phosphorylated at serine 780.

• References:

  Roesch A, et al.(2005) Mod Pathol. 18(4): 565-572. Huang H, et al.(2004)Oncogene. 23(12): 2161-2176. Kitagawa M, et al.(1996)EMBO J. 15(24): 7060-7069.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using Rb (phospho-Ser780) antibody (E011132).

Western blot analysis of extract from K562 cells untreated or treated with 10% serum after 48 hours of starvation, using Rb (Ab-780) antibody (E021110, Lane 1 and 2) and Rb (phospho-Ser780) antibody (E011132, Lane 3 and 4).