Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human syk around the phosphorylation site of tyrosine 323 (N-P-YP-E- P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human
SwissProtNo:
P43405
Applications:
IHC: 1:50~100
Specificity Sensitivity:
syk (phospho-Tyr323) Antibody detects endogenous levels of syk only when phosphorylated at tyrosine 323.
References:
Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447.
Turner, M. et al. (2000) Immunol. Today 21, 148-154.
Decker, M. et al. (1998) J. Biol. Chem. 273, 8867-8874.
Law, C.L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using syk (phospho-Tyr323) Antibody (E011546).