Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Tau around the phosphorylation site of threonine 205 (P-G-TP-P-G).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P10636
Applications:
WB: 1:500~1:1000 ELISA: 1:20000
Specificity Sensitivity:
Tau (phospho-Thr205) antibody detects endogenous levels of Tau only when phosphorylated at threonine 205.
References:
del C Alonso A, et al. (2004) J Biol Chem; 279(33): 34873-81.
Li G, Yin H, Kuret J (2004) J Biol Chem; 279(16): 15938-45.
Giasson BI, et al. (2002) Biochemistry; 41(51): 15376-87.
Liu F, et al. (2002) FEBS Lett; 530(1-3): 209.
Western blot analysis of extracts from mouse brain tissue using Tau (Ab-205) antibody (Line 1 and 2) and Tau (phospho- Thr205) antibody (Line 3 and 4).