• Catalog Number:

  E011540-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Serine 147 (A-F-SP-D-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P14635

• Applications:

  IHC: 1:50~100 IF:1:100~1:200

• Specificity Sensitivity:

  Cyclin B1 (phospho-Thr147) Antibody detects endogenous levels of Cyclin B1only when phosphorylated at Serine 147.

• References:

  Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470. Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685. Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.

Immunofluorescence staining of methanol-fixed HeLa cells using Cyclin B1 (phospho-Ser147) Antibody (E011540, Red).

P-Peptid - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1 (phospho-Ser147) Antibody (E011540).