• Catalog Number:

  E011008-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MEK2 around the phosphorylation site of threonine 394 (P-G-TP-P-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P36507

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200

• Specificity Sensitivity:

  MEK2 (phospho-Thr394) antibody detects endogenous levels of MEK2 only when phosphorylated at threonine 394.

• References:

  Crews C M, et al. (1992) Science. 258:478-480. Alessi D R, et al. (1994) EMBO J. 13:1610-1619. Rosen L B, et al. (1994) Neuron. 12:1207-1221.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using MEK2 (phospho-Thr394) antibody (E011008).

Western blot analysis of extracts from ovary cancer cells using MEK2 (phospho-Thr394) antibody (E011008).