Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year.
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human c-kit around the phosphorylation site of tyrosine 936 (H-I-YP-S-N).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human
SwissProtNo:
P10721
Applications:
IHC: 1:50~100
Specificity Sensitivity:
c-kit (phospho-Tyr936) Antibody detects endogenous levels of c-kit only when phosphorylated at tyrosine 936.
References:
Martin, F.H. et al. (1990) Cell 63, 203-11.
Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.
Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
Nocka, K. et al. (1990) EMBO J 9, 1805-13.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-kit (phospho-Tyr936) Antibody (E011539).