• Catalog Number:

  E1A7154B-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human MKK3 around the phosphorylation site of Serine189.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Rat

• SwissProtNo:

  P46734

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Specificity Sensitivity:

  MKK3 (Ab-189) Antibody detects endogenous levels of total MKK3 protein.

• References:

  Wang W, et al. (2002)Mol Cell Biol ; 22(10): 3389-403. Raingeaud J, et al. (1996) Mol Cell Biol; 16(3): 1247-55.

Immunohistochemical analysis of paraffin- embedded breast carcinoma, using MKK3(Ab-189) Antibody. Left: Untreated; Right: Treated with synthesized peptide.

Western blot analysis using MKK3(Ab-189) Antibody. Line1: The extracts from MDA-MB-435 cells; Line2: The extracts from MDA-MB-435 cells preincubated with synthesized peptide.