• Catalog Number:

  E011510-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Her3/ErbB3 around the phosphorylation site of tyrosine 1328 (P-D-YP-W-H).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P21860

• Applications:

  WB: 1:500~1:1000 IHC:1:50~1:100

• Specificity Sensitivity:

  Her3/ErbB3 (phospho-Tyr1328) Antibody detects endogenous levels of Her3/ErbB3 only when phosphorylated at tyrosine 1328.

• References:

  Holbro, T. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 8933-8938. Kobayashi, M. et al. (2003) Oncogene 22, 1294-1301. Kim, H.H. et al. (1994) J. Biol. Chem. 269, 24747-24755. Yarden, Y. and Sliwkowski, M.X. (2001) Nature Rev. Mol. Cell. Biol. 2, 127-137.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using Her3/ErbB3 (phospho-Tyr1328) Antibody (E011510).

Western blot analysis of extract from HUVEC cells using Her3/ErbB3 (Ab-1328) Antibody (E021510, Lane 1 and 2) and Her3/ErbB3 (phospho-Tyr1328) Antibody (E011510, Lane 3 and 4).