Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Ret around the phosphorylation site of tyrosine 1062 (K-L-YP-G-M).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P07949
Applications:
WB: 1:500~1:1000 ELISA: 1:10000
Alter Name:
C-ret, Proto-oncogene ret precursor, kinase Ret
Specificity Sensitivity:
Ret (phospho-Tyr1062) antibody detects endogenous levels of Ret only when phosphorylated at tyrosine 1062.
References:
Domenico Salvatore, Cancer Res., Feb 2001; 61: 1426 - 1431.
Hye Sook Jung, Mol. Endocrinol., Nov 2005; 19: 2748 - 2759.
Iván Plaza-Menacho, J. Biol. Chem., Mar 2007; 282: 6415 - 6424.
Matthias Drosten, J Natl Cancer Inst, Aug 2004; 96: 1231 - 1239.
All Sites:
Human: Tyr1062; Mouse: Tyr1063; Rat: Tyr1064
Western blot analysis of extracts from K562 cells, using Ret (phospho-Tyr1062) antibody.