Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human
cofilin1 around the phosphorylation site of tyrosine 139 (N-C-YP-E-E).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed
by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P23528
Applications:
IHC: 1:50~100
Specificity Sensitivity:
cofilin1 (phospho-Tyr139) Antibody detects endogenous levels of cofilin1 only when
phosphorylated at tyrosine 139.
References:
Carlier, M. et al. (1999) J. Biol. Chem. 274, 33827-33830.
Arber, S. et al. (1998) Nature 393, 805-809.
Yang, N. et al. (1998) Nature 393, 809-812.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using cofilin1 (phospho-Tyr139) Antibody (E011506).