• Catalog Number:

  E011282-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Integrin β3 around the phosphorylation site of tyrosine 785 (I-T-YP-R-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P05106

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  Integrin β3 (Phospho-Tyr785) antibody detects endogenous levels of Integrin β3 only when phosphorylated at tyrosine 785.

• References:

  Sujoy Bhattacharya, et al. (2006) Biochem J. August 1; 397(Pt 3): 437–447 Courter DL, et al. (2005) J Biol Chem Apr 01; 280(13): 12145-12151 Chandhoke SK, et al. (2004) J Cell Sci Mar 15; 117(Pt 8): 1431-1441

Western blot analysis of extract from A431 cells untreated or treated with Insulin (40μg/ml, 15min), using Integrin β3 (phospho-Tyr785) antibody (E011282, Lane 1 and 2).