• Catalog Number:

  E011502-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human AKT1 around the phosphorylation site of threonine 450 (T-I-TP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P31749

• Applications:

  IHC: 1:50~100 IF:1:100~1:200

• Specificity Sensitivity:

  AKT1 (phospho-Thr450) Antibody detects endogenous levels of AKT1 only when phosphorylated at threonine 450.

• References:

  Franke, T.F. et al. (1997) Cell 88, 435-7. Sarbassov, D.D. et al. (2005) Science 307, 1098-101. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5. Hajduch, E. et al. (2001) FEBS Lett 492, 199-203. Gesbert, F. et al. (2000) J Biol Chem 275, 39223-30.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1 (phospho- Thr450) Antibody (E011502).

Immunofluorescence staining of methanol-fixed HeLa cells using AKT1 (phospho-Thr450) Antibody (E011502, red).