• Catalog Number:

  E1A8025A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of serine 1071 (Y-S-SP-D-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%);Rat (Identities = 85%, Positives = 85%)

• SwissProtNo:

  P00533

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  EC 2.7.10.1; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor precursor; ERBB1; kinase EGFR; Receptor protein-tyrosine kinase ErbB-1

• Specificity Sensitivity:

  EGFR (Phospho-Ser1071) antibody detects endogenous levels of EGFR only when phosphorylated at serine 1071.

• References:

  Ullrich A., Nature 309:418-425(1984). Ilekis J.V., Mol. Reprod. Dev. 41:149-156(1995). Reiter J.L., Nucleic Acids Res. 24:4050-4056(1996).

• All Sites:

  Human: Ser1071; Mouse: Ser1071; Rat: Ser1071

Western blot analysis of extracts from HepG2 cells, treated with serum (20%, 15mins), using EGFR (Phospho-Ser1071) antibody.