Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human TrkB around the phosphorylation site of tyrosine 705 (T-D-Y P-Y-R).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q16620
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
TrkB (Phospho-Tyr705) Antibody detects endogenous levels of TrkB only when phosphorylated at tyrosine 705.
References:
Woronowicz A, et al. Glycobiology. 2007 Jan;17(1):10-24.
Mojsilovic-Petrovic J, et al. J Neurosci. 2006 Sep 6;26(36):9250-63.
Lewis MA, et al. Mol Pharmacol. 2006 Apr;69(4):1396-404.
Cai D, et al. Physiol Genomics. 2006 Feb 14;24(3):191-7.
Western blot analysis of extracts from mouse brain tissue, using TrkB (Phospho-Tyr705) antibody (E011328, Lane 1 and 2).