• Catalog Number:

  E011230-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 636 (P-M-SP-P-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35568

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100

• Specificity Sensitivity:

  IRS-1 (phospho-Ser636) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 636.

• References:

  Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645 Tzatsos A, et al. (2006) Mol Cell Biol; 26(1): 63-76 Kadowaki T, et al. (2000) J Clin Invest; 106(4): 459-465 Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645 Szanto I, et al. (2000) Proc Natl Acad Sci U S A; 97(5): 2355-2360

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using IRS-1 (phospho- Ser636) antibody (E011230).

Western blot analysis of extracts from 293 cells treated with insulin(100nM, 30min) or PMA (0.2μM, 15min) using IRS-1 (Ab-636) antibody (E021223, Lane 1, 2 and 3) and IRS-1 (phospho-Ser636) antibody (E011230, Lane 4, 5 and 6).