• Catalog Number:

  E011323-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Smad2 around the phosphorylation site of threonine 220 (P-E-TP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q15796

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  Smad2 (Phospho-Thr220) Antibody detects endogenous levels of Smad2 only when phosphorylated at threonine 220.

• References:

  Sang Gyun Kim et al. (2004) Mol Biol Cell. February; 15(2): 420–434. Rebecca A. Randall et al. (2004) Mol Cell Biol. February; 24(3): 1106–1121. Kimberly A Brown et al. (2004) Breast Cancer Res.; 6(3): R215–R231.

Western blot analysis of extract from Hela cells untreated or treated with IL-4 (100ng/mL, 30min), using Smad2 (Ab-220) Antibody (E021323, Lane 1 and 2) and Smad2 (Phospho- Thr220) Antibody (E011323, Lane 3 and 4).