• Catalog Number:

  E1A8135A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MAP2K1 around the phosphorylation site of threonine 386 (P-S-TP-P-T).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q02750

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK1; MP2K1; PRKMK1; kinase MEK1

• Specificity Sensitivity:

  MAP2K1 (Phospho-Thr386) antibody detects endogenous levels of MAP2K1 only when phosphorylated at threonine 386.

• References:

  Seger R., J. Biol. Chem. 267:25628-25631(1992). Zheng C.-F., J. Biol. Chem. 268:11435-11439(1993). Zheng C.-F., EMBO J. 13:1123-1131(1994).

• All Sites:

  Human: Thr386; Mouse: Thr386; Rat: Thr386

Western blot analysis of extracts from A549 cells and RAW264.7 cells treated with UV, and NIH/3T3 cells treated with EGF, using MAP2K1 (Phospho-Thr386) antibody.