Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human
IKK-β around the phosphorylation site of tyrosine 199 (Q-K-YP-T-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed
by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
O14920
Applications:
IF:1:100~1:200
Specificity Sensitivity:
IKK-β (Phospho-Tyr199) Antibody detects endogenous levels of IKK-βonly when
phosphorylated at tyreonine 199.
References:
Chen RA, et al. PLoS Pathog. 2008 Feb 8;4(2):e22
Yen CJ,et al. Cancer Res. 2008 Apr 15;68(8):2632-40
Immunofluorescence staining of methanol-fixed HeLa cells using IKK-β (Phospho-Tyr199) Antibody (E011305, Red).