• Catalog Number:

  E011179-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ASK1 around the phosphorylation site of serine 966 (S-I-SP-L-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q99683

• Applications:

  WB:1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  ASK1 (phospho-Ser966) antibody detects endogenous levels of ASK1 only when phosphorylated at serine 966.

• References:

  Zhang W, et al. (2005) J Biol Chem. 280(19): 19036-19044. Fujii K, et al. (2004) Oncogene. 23(29):5099-5104. Goldman EH, et al. (2004) J Biol Chem 2004 Mar 12; 279(11): 10442-10449. Zhang L, et al. (1999) Proc Natl Acad Sci U S A. 96(15): 8511-8515.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ASK1 (phospho- Ser966) antibody (E011179).

Western blot analysis of extract from 3T3 cells treated with 10% serum after 24 hours of starvation, using ASK1 (phospho-Ser966) antibody (E011179, Lane 1 and 2).